superscript iii first-strand synthesis system 18080051 (Thermo Fisher)
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superscript iii first-strand synthesis system 18080051
Superscript Iii First Strand Synthesis System 18080051, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+first-strand+synthesis+system+18080051/high+capacity+cdna+reverse+transcription+kit/bio_rxiv__2025__07__15__664932-209-18-24
Average 90 stars, based on 1 article reviews
Superscript Iii First Strand Synthesis System 18080051, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+first-strand+synthesis+system+18080051/high+capacity+cdna+reverse+transcription+kit/bio_rxiv__2025__07__15__664932-209-18-24
Average 90 stars, based on 1 article reviews
superscript iii first-strand synthesis system 18080051 - by Bioz Stars,
2026-10
90/100 stars
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Reverse Transcription:Article Title: Neuronal substance-P drives breast cancer growth and metastasis via an extracellular RNA-TLR7 axis Article Snippet: RNA was extracted from cells using the RNA purification kit (Norgen Biotek, 37500)), according to the manufacturer’s protocol. .. Reverse transcription (RT-PCR) was performed on 2-5 μg of RNA using the Article Title: Neuronal substance-P drives metastasis via an extracellular RNA/TLR7 axis Article Snippet: RNA was extracted from cells using the RNA purification kit (Norgen Biotek, 37500), according to the manufacturer’s protocol. .. Reverse transcription (RT-PCR) was performed on 2–5 μg of RNA using the Article Title: A double-agent microRNA regulates viral cross-kingdom infection in animals and plants Article Snippet: RNA quality was detected by a NanoDrop One (Thermo Scientific, Waltham, MA; 840-317400) and gel electrophoresis. .. For mRNA or viral RNA qPCR, 2 μg of RNA was reverse transcribed to cDNA by using the Article Title: Deletion upstream of MAB21L2 highlights the importance of evolutionarily conserved non-coding sequences for eye development Article Snippet: For qRT-PCR expression assays, at least three independent RNA samples per developmental stage were extracted from whole embryos (10–12 pooled embryos per sample), dissected embryonic eyes (30–40 pooled eyes per sample) and midbrains (20–25 pooled brains per sample) using Direct-zol RNA MiniPrep kit (Zymo Research, cat#R2052). .. To remove DNA contamination, 1 μg of RNA was digested with 1 unit of DNaseI (ThermoFisher, cat# EN0523) for 15 min at room temperature (RT) and inactivated in the presence of 2.5 mM EDTA for 10 min at 65 ◦ C. Reverse transcription was performed using the Article Title: Bidirectional control of a metabolic transition by the GID ubiquitin ligase Article Snippet: .. Contaminating genomic DNA was removed using the RNase-Free DNase Set (79254, Qiagen), followed by reverse transcription with the other:Article Title: A commonly inherited human PCSK9 germline variant drives breast cancer metastasis via LRP1 receptor. Article Snippet: Article A commonly inherited human PCSK9 germline variant drives breast cancer metastasis via LRP1 receptor Polymerase Chain Reaction:Article Title: The contribution of de novo coding mutations to meningomyelocele. Article Snippet: RNA was extracted from patient and control fibroblast cells using TRIzol reagent (ThermoFisher Scientific, 15596026) following the manufacturer’s guidelines. .. The extracted RNA was used to synthesize cDNA using the Real-time Polymerase Chain Reaction:Article Title: A double-agent microRNA regulates viral cross-kingdom infection in animals and plants Article Snippet: RNA quality was detected by a NanoDrop One (Thermo Scientific, Waltham, MA; 840-317400) and gel electrophoresis. .. For mRNA or viral RNA qPCR, 2 μg of RNA was reverse transcribed to cDNA by using the Incubation:Article Title: Discovery of the first-in-class DOT1L PROTAC degrader. Article Snippet: DOT1L is the lysine methyltransferase responsible for histone H3 lysine 79 (H3K79) methylation and plays a crucial role in leukemia progression.. Furthermore, DOT1L has biological functions that are independent of its methyltransferase activity.. Therefore, targeting and degrading DOT1L with PROteolysis TArgeting Chimeras (PROTACs) could represent a promising therapeutic strategy. cDNA Synthesis:Article Title: Discovery of the first-in-class DOT1L PROTAC degrader. Article Snippet: DOT1L is the lysine methyltransferase responsible for histone H3 lysine 79 (H3K79) methylation and plays a crucial role in leukemia progression.. Furthermore, DOT1L has biological functions that are independent of its methyltransferase activity.. Therefore, targeting and degrading DOT1L with PROteolysis TArgeting Chimeras (PROTACs) could represent a promising therapeutic strategy. |